583 RNA sequencing of immortalised balding and non balding DPCs identifies potential balding gene signatures

Androgenic alopecia affects hair follicles from frontal and vertex area. Hair inductive properties of DPCs are lost in bi-dimentional (2D) culture. In a recent study three dimentional (3D) structure cultured was able to recapitulate 22% molecular signature the intact dermal papilla The aim this compare gene profile expression immortalised balding (B-DPCs) non-balding (NB-DPCs) 3 different culture conditions: bi-dimensional (2D), dimensional co-culture with adipose derived stem cells (ASCs). We also compared primary same conditions early, mid late passages (P3, P6 P9). Immortalised P3, P9 were used for 2D culture, 3D spheroids co ASC spheroids. RNA sequencing performed -with14 million reads/ sample alignment Human build HG 38 genome assembly- then Seqmonk software analysis differentially expressed genes (should I put Babraham institute?) clustering by using David performed. Overall showed that B, NB DPC cluster very distinctly. ASCs create changes all types. Changes more similar between distinct change DPCs. B-DPCs present an inflammatory which does not significantly ASCs. extra cellular matrix organisation features NB-DPCs emphasized as well promotion few inhibitory processes on inflammation. emphasize maintenance immortalisation conclusion, is non promote pathways conditions.